U-74389G可抑制大鼠局灶性脑缺血再灌注后的脂质过氧化和细胞凋亡。

U-74389G suppresses lipid peroxidation and apoptosis following focal cerebral ischemia and reperfusion in rats.

作者信息

Zhang F, Zhou L, Huang F, Liang Y, Zhang G

机构信息

Department of Neurosurgery, Huashan Hospital, Shanghai Medical University, Shanghai 200040, China.

出版信息

Chin Med J (Engl). 1998 Sep;111(9):838-41.

PMID:11155678

Abstract

OBJECTIVE

To study the antioxidant and antiapoptotic effects of U-74389G on ischemic brain in rats.

METHODS

Right focal cerebral ischemia and reperfusion was induced by suturing in 103 male SD rats weighing 280-310 g. To determine LPO in the cortex and striatum, we randomly divided 78 rats into 5 groups: group 1, sham-operative group (6 rats); group 2, control (18); group 3, U-74389G administration before ischemia (18); group 4, U-74389G administration before reperfusion; group 5, CS-4 vehicle as negative control. Groups 2-5 were further divided into 3 subgroups according to the duration of reperfusion. Another 25 rats were similarly grouped (5) to detect apoptosis with TUNEL stain.

RESULTS

The concentrations of MDA in groups 3 and 4 were reduced and the activities of SOD and concentrations of GSH were partially restored as compared with the control group. These effects were more significant in group 3 than in group 4. Otherwise, CS-4 can not provide protective effects. The number of apoptotic cells in groups 3 (P < 0.01) and 4 (P < 0.05) was significantly reduced as compared with the control group.

CONCLUSIONS

U-74389G has antioxidant and antiapoptotic effects on ischemic brain in rats. The administration of U-74389G before the onset of ischemia was more useful than that before the onset of reperfusion.

摘要

目的

研究U-74389G对大鼠缺血性脑损伤的抗氧化及抗凋亡作用。

方法

采用线栓法对103只体重280 - 310 g的雄性SD大鼠进行右侧局灶性脑缺血再灌注。为测定皮质和纹状体中的脂质过氧化物（LPO），将78只大鼠随机分为5组：第1组，假手术组（6只大鼠）；第2组，对照组（18只）；第3组，缺血前给予U-74389G组（18只）；第4组，再灌注前给予U-74389G组；第5组，CS-4溶媒作为阴性对照组。第2 - 5组根据再灌注时间进一步分为3个亚组。另外25只大鼠按同样方法分组（每组5只），采用TUNEL染色检测细胞凋亡情况。