[利用人工异嗜性诊断法检测忽视罗蛉、强壮罗蛉和侵扰锥猎蝽（半翅目，猎蝽科，锥猎蝽亚科）对两种克氏锥虫菌株（动基体目，锥虫科）感染的易感性]

[Susceptibility of Rhodnius neglectus, Rhodnius robustus and Triatoma infestans (Hemiptera, Reduviidae, Triatominae) to infection by two strains of Trypanosoma cruzi (Kinetoplastidae, Trypanosomatidae) using artificial xenodiagnosis].

作者信息

Martins L P, da Rosa J A, Castanho R E, Sauniti G L, Medeiros H

机构信息

Disciplina de Parasitologia, Faculdade de Medicina de Marília, Av. Monte Carmelo 800, 17519-030, Marília, SP, Brazil.

出版信息

Rev Soc Bras Med Trop. 2000 Nov-Dec;33(6):559-63. doi: 10.1590/s0037-86822000000600007.

DOI:10.1590/s0037-86822000000600007

PMID:11175586

Abstract

The susceptibility of 3rd instar nymph of Triatominae Rhodnius neglectus, R. robustus and Triatoma infestans to Trypanosoma cruzi Y and AMJM strains was verified using artificial xenodiagnosis. After the accomplishment of the xenodiagnosis, the faeces of the Triatominae were analyzed on two-day intervals from day 5 until day 31 post infection, using the abdominal compression technique. The results showed differences in the susceptibility of the Triatominae for the two strains studied, and the optimal period reading differed from day 11 to day 19 for the Y strain and from day 11 to day 15 for the AMJM strain. For the Y strain, all three Triatominae species showed good susceptibility, whereas in the AMJM strain, the highest susceptibility was observed with R. neglectus, followed by T. infestans and R. robustus.

摘要

利用人工接种诊断法，验证了克氏锥蝽属的忽视罗蝽、强壮罗蝽和骚扰锥蝽三龄若虫对克氏锥虫Y株和AMJM株的易感性。人工接种诊断完成后，从感染后第5天到第31天，每隔两天使用腹部挤压技术分析锥蝽的粪便。结果显示，所研究的两种菌株对锥蝽的易感性存在差异，Y株的最佳读数期为第11天至第19天，AMJM株为第11天至第15天。对于Y株，所有三种锥蝽均表现出良好的易感性，而在AMJM株中，忽视罗蝽的易感性最高，其次是骚扰锥蝽和强壮罗蝽。