使用多重聚合酶链反应鉴定卡介苗疫苗的亚菌株。

Identification of substrains of BCG vaccine using multiplex PCR.

作者信息

Bedwell J, Kairo S K, Behr M A, Bygraves J A

机构信息

Division of Bacteriology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Hertfordshire, EN6 3QG, Potters Bar, UK.

出版信息

Vaccine. 2001 Feb 28;19(15-16):2146-51. doi: 10.1016/s0264-410x(00)00369-8.

DOI:10.1016/s0264-410x(00)00369-8

PMID:11228387

Abstract

Current methods for determining the identity of substrains of Mycobacterium bovis BCG (BCG) vaccine are labour intensive, or provide only limited substrain differentiation. In this paper we describe a multiplex PCR that distinguishes between M. tuberculosis (TB) and M. bovis and the non-pathogenic BCG strain, and also subdivides the BCG vaccine substrains investigated into seven distinct fingerprints based on six target regions in the DNA. This test is specific, rapid, reproducible and portable and is proposed as a novel test for BCG vaccine control. It offers substantial advantages over the methods currently in use. Using this test we have characterised a number of commercial BCG vaccines.

摘要

目前用于确定卡介苗（BCG）疫苗亚菌株身份的方法劳动强度大，或者只能提供有限的亚菌株区分。在本文中，我们描述了一种多重PCR方法，该方法可区分结核分枝杆菌（TB）和牛分枝杆菌以及非致病性卡介苗菌株，并且还能根据DNA中的六个靶区域将所研究的卡介苗疫苗亚菌株细分为七个不同的指纹图谱。该检测方法具有特异性、快速、可重复和便携的特点，被提议作为一种用于卡介苗疫苗质量控制的新型检测方法。与目前使用的方法相比，它具有显著优势。我们使用该检测方法对多种市售卡介苗疫苗进行了特征分析。