Wilde A, Lizarraga S B, Zhang L, Wiese C, Gliksman N R, Walczak C E, Zheng Y
Howard Hughes Medical Institute, Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA.
Nat Cell Biol. 2001 Mar;3(3):221-7. doi: 10.1038/35060000.
The guanosine tri-phosphatase Ran stimulates assembly of microtubule spindles. However, it is not known what aspects of the microtubule cytoskeleton are subject to regulation by Ran in mitosis. Here we show that Ran-GTP stimulates microtubule assembly by increasing the rescue frequency of microtubules three- to eightfold. In addition to changing microtubule dynamics, Ran-GTP also alters the balance of motor activities, partly as a result of an increase in the amount of motile Eg5, a plus-end-directed microtubule motor that is essential for spindle formation. Thus, Ran regulates multiple processes that are involved in spindle assembly.
鸟苷三磷酸酶Ran刺激微管纺锤体的组装。然而，在有丝分裂过程中，微管细胞骨架的哪些方面受Ran调控尚不清楚。在这里我们表明，Ran-GTP通过将微管的拯救频率提高三到八倍来刺激微管组装。除了改变微管动力学外，Ran-GTP还改变了马达活性的平衡，部分原因是运动性Eg5（一种对纺锤体形成至关重要的正端定向微管马达）数量的增加。因此，Ran调节纺锤体组装中涉及的多个过程。
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