[缺氧预处理对培养的大鼠海马神经元缺氧耐受性及白细胞介素-1β表达的影响]
[Effects of hypoxic preconditioning on anoxic tolerance and IL-1 beta expression in cultured rat hippocampal neurons].
作者信息
Ding A S, Wang F Z, Yu M, Fan M
机构信息
Department of Neurobiology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2000 Nov;16(4):294-7.
AIM
To study the effects of hypoxic-preconditioning on anoxia-tolerance and IL-1 beta expression in cultured rat hippocampal neurons after anoxia.
METHODS
12 day cultured hippocampal neurons in control and hypoxic-preconditioning group were exposed to anoxic environment(0.9 L/LN2 + 0.1 L/LCO2) for 2, 4, 8 and 12 h. The neurons were immunocytochemically stained using the anti-serum against rhIL-1 beta, The number of survival neurons and the mean optical density (OD) of IL-1 beta expression were investigated.
RESULTS
The morphological changes and IL-1 beta expression induced by acute anoxia in hypoxic-preconditioning neurons were significantly less than those in control. The number of survival neurons was higher in the hypoxia-preconditioning group than that in control group after acute hypoxia.
CONCLUSION
Hypoxia-preconditioning can induce the development of anoxia-tolerance in cultured hippocampal neurons. The decreased IL-1 beta in hippocampal neurons may be an adaptive reaction to acute anoxia.
目的
研究缺氧预处理对培养的大鼠海马神经元缺氧耐受性及缺氧后白细胞介素-1β（IL-1β）表达的影响。
方法
将培养12天的对照组和缺氧预处理组海马神经元置于缺氧环境（0.9 L/L N₂ + 0.1 L/L CO₂）中2、4、8和12小时。用抗重组人IL-1β抗血清对神经元进行免疫细胞化学染色，观察存活神经元数量及IL-1β表达的平均光密度（OD）。
结果
缺氧预处理神经元急性缺氧诱导的形态学变化及IL-1β表达明显少于对照组。急性缺氧后，缺氧预处理组存活神经元数量高于对照组。
结论
缺氧预处理可诱导培养的海马神经元产生缺氧耐受性。海马神经元中IL-1β的降低可能是对急性缺氧的一种适应性反应。
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