[Adaptation of diaphragm muscle strip mechanics to chronic electrical stimulation and the effect of change in extracellular Ca2+ in rabbit].

AIM

The mechanical character of diaphragm muscle after chronic electrical stimulation(CES) and effect of the extracellular Ca2+ change have not yet been explored. We wondered whether there might be great different change and effect on muscle mechanics after CES and the extracellular Ca2+ change.

METHODS

The twitch tension(Pt), time to peak tension(TPT), half-relaxation time(1/2 RT), tetanic tension(Po), fatigue index (FI) and fatigue recovery index(FRI) were respectively measured in normal group and CES groups; the switch tensions of diaphragm muscle strips were observed in the standard Hank's solution and the Hank's with free Ca2+.

RESULTS

There were more significant decrease in Pt, Po, FI and FRI, more significant lengthening in TPT and 1/2 RT in 10 Hz and 20 Hz groups(P < 0.01). However, there was completely opposite effect in 50 Hz and 100 Hz groups. There were more significant effect on muscle mechanics of contraction and relaxation in 10 Hz and 20 Hz groups than that in 50 Hz and 100 Hz groups when the extracellular Ca2+ changed.

CONCLUSION

After CES the significantly frequency dependent were presented on mechanical character of diaphragm muscle strips, and there were more effect on diaphragm muscle mechanics in 10 Hz and 20 Hz groups when the extracellular Ca2+ was changed.