补充氧气可控制兔血管移植物与动脉吻合处的细胞增殖和吻合口内膜增生。

Supplemental oxygen controls cellular proliferation and anastomotic intimal hyperplasia at a vascular graft-to-artery anastomosis in the rabbit.

作者信息

Lee E S, Caldwell M P, Tretinyak A S, Santilli S M

机构信息

Department of Surgery, University of Minnesota and the Veterans Affairs Medical Center, MN 55417, USA.

出版信息

J Vasc Surg. 2001 Mar;33(3):608-13. doi: 10.1067/mva.2001.113495.

DOI:10.1067/mva.2001.113495

PMID:11241134

Abstract

PURPOSE

The purpose of this study was to determine whether the administration of 40% supplemental oxygen (O ( 2) ) will decrease cellular proliferation and intimal hyperplasia (IH) at a prosthetic vascular graft (PVG)-to-artery anastomosis.

METHODS

Twenty New Zealand white rabbits underwent placement of a 3-mm polytetrafluoroethylene graft in their infrarenal aorta. Four groups of five rabbits were placed either in a normoxic (21%) environment or in a 40% supplemental O ( 2) environment for 7 or 42 days. Twenty-four hours before the rabbits were humanely killed for aortic graft harvest, BrDU (5-bromo-2'-deoxyuridine) was injected into the rabbits intraperitoneally. Image analysis (Bioquant) morphometrics were used to measure cells with BrDU staining and intimal areas at the distal anastomosis. Cellular proliferation is defined as positively staining BrDU cells divided by all cells in the artery wall. IH is reported as a ratio between the intimal area and the medial area to standardize the varying aortic size and degree of aortic fixation among rabbits. The Student t test was used to compare cellular proliferation and IH between control and O ( 2) -treated rabbits.

RESULTS

Cellular proliferation in the intima at 7 days was significantly reduced in the O ( 2) -treated animals (1.7% +/- 1%) versus the control animals (28.6% +/- 3%) ( P =.0001). The cellular proliferation in the intima at 42 days returned to preoperative levels in the O ( 2) -treated group (0.15%) and in the control group (0.11%) ( P = not significant). IH at 7 days was minimal, and no difference between the O ( 2) -treated group (0.017 +/-.006) and the control group (0.009 +/-.03) ( P = not significant) was found. IH was significantly reduced at 42 days in the O ( 2) -treated animals (0.031 +/-.012) when compared with the control animals (0.193 +/-.043) ( P =.006).

CONCLUSIONS

Supplemental O ( 2) (40%) significantly reduces cellular proliferation and IH at the distal anastomosis of a PVG-to-artery anastomosis in the rabbit model.

摘要

目的

本研究旨在确定给予40%的补充氧气（O₂）是否会减少人工血管移植物（PVG）与动脉吻合处的细胞增殖和内膜增生（IH）。

方法

20只新西兰白兔在其肾下腹主动脉植入3毫米的聚四氟乙烯移植物。将四组，每组五只兔子，分别置于常氧（21%）环境或40%补充O₂环境中7天或42天。在对兔子实施安乐死以获取主动脉移植物前24小时，向兔子腹腔内注射溴脱氧尿苷（BrDU）。采用图像分析（Bioquant）形态计量学方法测量BrDU染色的细胞以及远端吻合处的内膜面积。细胞增殖定义为BrDU阳性染色细胞数除以动脉壁内的所有细胞数。IH报告为内膜面积与中膜面积之比，以标准化不同兔子之间主动脉大小和主动脉固定程度的差异。采用Student t检验比较对照组和O₂处理组兔子之间的细胞增殖和IH情况。

结果

与对照组动物（28.6%±3%）相比，O₂处理组动物在7天时内膜中的细胞增殖显著降低（1.7%±1%）（P = 0.0001）。在42天时，O₂处理组（0.15%）和对照组（0.11%）内膜中的细胞增殖均恢复到术前水平（P = 无显著性差异）。7天时IH最小，未发现O₂处理组（0.017±0.006）与对照组（0.009±0.03）之间存在差异（P = 无显著性差异）。与对照组动物（0.193±0.043）相比，O₂处理组动物在42天时IH显著降低（0.031±0.012）（P = 0.006）。