中性粒细胞呼吸爆发氧化酶的组装：p67PHOX与细胞色素b558之间的直接相互作用。
Assembly of the neutrophil respiratory burst oxidase: a direct interaction between p67PHOX and cytochrome b558.
作者信息
Dang P M, Cross A R, Babior B M
机构信息
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
出版信息
Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3001-5. doi: 10.1073/pnas.061029698.
Activation of the phagocyte NADPH oxidase complex requires the assembly of the cytosolic factors p47(PHOX), p67(PHOX), p40(PHOX), and Rac1 or Rac2, with the membrane-bound cytochrome b(558). Whereas the interaction of p47(PHOX) with cytochrome b(558) is well established, an interaction between p67(PHOX) and cytochrome b(558) has never been investigated. We report here a direct interaction between p67(PHOX) and cytochrome b(558). First, labeled p67(PHOX) recognizes a 91-kDa band in specific granules from a normal patient but not from a cytochrome b(558)-deficient patient. Second, p67(PHOX) binds to cytochrome b(558) that has been bound to nitrocellulose. Third, GTP-p67(PHOX) bound to glutathione agarose is able to pull down cytochrome b(558.) Rac1-GTP or Rac1-GDP increased the binding of p67(PHOX) to cytochrome b(558), suggesting that at least one of the oxidase-related functions of Rac1 is to promote the interaction between p67(PHOX) and cytochrome b(558).
吞噬细胞NADPH氧化酶复合物的激活需要胞质因子p47（PHOX）、p67（PHOX）、p40（PHOX）和Rac1或Rac2与膜结合的细胞色素b（558）组装在一起。虽然p47（PHOX）与细胞色素b（558）之间的相互作用已得到充分证实，但p67（PHOX）与细胞色素b（558）之间的相互作用从未被研究过。我们在此报告p67（PHOX）与细胞色素b（558）之间存在直接相互作用。首先，标记的p67（PHOX）能识别正常患者特异性颗粒中的一条91 kDa条带，但不能识别细胞色素b（558）缺陷患者的该条带。其次，p67（PHOX）与已结合到硝酸纤维素上的细胞色素b（558）结合。第三，结合到谷胱甘肽琼脂糖上的GTP-p67（PHOX）能够拉下细胞色素b（558）。Rac1-GTP或Rac1-GDP增加了p67（PHOX）与细胞色素b（558）的结合，这表明Rac1的至少一种氧化酶相关功能是促进p67（PHOX）与细胞色素b（558）之间的相互作用。
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