对在日本和肯尼亚分离出的人轮状病毒4型（G4）的VP7、VP4和NSP4基因进行分子特征分析。

Molecular characterization in the VP7, VP4 and NSP4 genes of human rotavirus serotype 4 (G4) isolated in Japan and Kenya.

作者信息

Kudo S, Zhou Y, Cao X R, Yamanishi S, Nakata S, Ushijima H

机构信息

Department of Pediatrics, Teikyo University School of Medicine, Tokyo, Japan.

出版信息

Microbiol Immunol. 2001;45(2):167-71. doi: 10.1111/j.1348-0421.2001.tb01286.x.

DOI:10.1111/j.1348-0421.2001.tb01286.x

PMID:11293484

Abstract

The VP7, VP4 and NSP4 genes of human rotavirus serotype 4 (G4) were analyzed to investigate intraserotypic variations. The techniques used included reverse transcription polymerase chain reaction with subtype specific primers, restriction fragment length polymorphism analysis and sequence analysis. Twelve isolates (nine from Japan and three from Kenya) and two standard strains (Hochi, Odelia) were G4A P[8] Wa group NSP4. A standard strain (ST3) was G4A P[6] Wa group NSP4 and a strain (VA70) was G4B P[8] Wa group NSP4. These results show G4 rotaviruses can be divided into three combinations at the moment.

摘要

分析了人轮状病毒4型（G4）的VP7、VP4和NSP4基因，以研究血清型内变异。所使用的技术包括使用亚型特异性引物的逆转录聚合酶链反应、限制性片段长度多态性分析和序列分析。12株分离株（9株来自日本，3株来自肯尼亚）和2株标准株（Hochi、Odelia）为G4A P[8] Wa组NSP4。1株标准株（ST3）为G4A P[6] Wa组NSP4，1株毒株（VA70）为G4B P[8] Wa组NSP4。这些结果表明，目前G4轮状病毒可分为三种组合。