神经降压素受体亚型NTR3参与神经降压素对癌细胞系的生长作用。
Involvement of the neurotensin receptor subtype NTR3 in the growth effect of neurotensin on cancer cell lines.
作者信息
Dal Farra C, Sarret P, Navarro V, Botto J M, Mazella J, Vincent J P
机构信息
Institut de Pharmacologie Moléculaire et Cellulaire, Sophia-Antipolis, Valbonne, France.
出版信息
Int J Cancer. 2001 May 15;92(4):503-9. doi: 10.1002/ijc.1225.
The expression of the 3 currently known neurotensin receptors was studied in human cancer cells of prostatic, colonic or pancreatic origin by means of RT-PCR analysis and binding experiments. All the cells selected for this work have been shown to exhibit a growth response to neurotensin. We found that the 7 transmembrane domain, levocabastine insensitive receptor (NTR1) is expressed in most but not all of the cells studied whereas the 7 transmembrane domain, levocabastine sensitive receptor (NTR2) is present in none of these cells. The 100 kDa-type I neurotensin receptor (NTR3) is expressed in all the cells assayed. Moreover, we demonstrated that neurotensin can stimulate the growth of CHO cells stably transfected with the NTR3. Taken together, our results strongly suggest that the NTR3 subtype could be involved in the growth response of human cancer cells to neurotensin.
通过逆转录聚合酶链反应（RT-PCR）分析和结合实验，研究了目前已知的3种神经降压素受体在前列腺、结肠或胰腺来源的人类癌细胞中的表达情况。所有选用于此项研究的细胞均已证实对神经降压素表现出生长反应。我们发现，7跨膜结构域、左卡巴斯汀不敏感受体（NTR1）在大多数但并非所有研究的细胞中表达，而7跨膜结构域、左卡巴斯汀敏感受体（NTR2）在这些细胞中均未出现。100 kDa I型神经降压素受体（NTR3）在所有检测的细胞中均有表达。此外，我们还证明神经降压素可刺激稳定转染了NTR3的CHO细胞的生长。综合来看，我们的结果强烈表明NTR3亚型可能参与了人类癌细胞对神经降压素的生长反应。
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