Zhu Q, Fan M, Bian Z, Chen Z, Zhang Q, Peng B
School of Stomatology, Hubei Medical University, Wuhan, 430079, P. R. China.
Chin J Dent Res. 2000 Aug;3(2):21-5.
The aim of this study is to investigate the temporal and spatial expression and localization of transforming growth factor-beta 1 mRNA during mouse tooth development.
The distribution pattern of TGF-beta 1 mRNA during tooth development was analyzed on 5 microns serial sections of paraformaldehyde-fixed and paraffin-embedded embryonic mouse heads or mandibles.
The results show that TGF-beta 1 mRNA is expressed during mouse tooth development in a temporally and spatially regulated fashion. Local expression of TGF-beta 1 mRNA in the dental epithelium at budstaged (E13) and capstaged (E15) teeth were observed. During bell stage (E16-18), TGF beta 1 mRNA was very abundant in the ameloblast layer and dental papilla cells. The expression of TGF-beta 1 increased in the layer of odontoblast and ameloblast with the differentiation of these cells.
These results suggest that TGF-beta 1 may have an important role in odontogenesis and that it acts as a paracrine and autoinducing factor.
本研究旨在探讨转化生长因子-β1 mRNA在小鼠牙齿发育过程中的时空表达及定位。
在经多聚甲醛固定、石蜡包埋的胚胎小鼠头部或下颌骨的5微米连续切片上，分析牙齿发育过程中TGF-β1 mRNA的分布模式。
结果表明，TGF-β1 mRNA在小鼠牙齿发育过程中以时空调控的方式表达。在芽期（E13）和帽期（E15）牙齿的牙上皮中观察到TGF-β1 mRNA的局部表达。在钟状期（E16 - 18），TGF-β1 mRNA在成釉细胞层和牙乳头细胞中非常丰富。随着成牙本质细胞和成釉细胞的分化，TGF-β1的表达在这些细胞层中增加。
这些结果表明，TGF-β1可能在牙齿发生中起重要作用，并且它作为旁分泌和自诱导因子发挥作用。
