利用毛细管电泳法对脂质体进行的研究。

Study on liposomes by capillary electrophoresis.

作者信息

Wiedmer S K, Hautala J, Holopainen J M, Kinnunen P K, Riekkola M L

机构信息

Department of Chemistry, University of Helsinki, Finland.

出版信息

Electrophoresis. 2001 Apr;22(7):1305-13. doi: 10.1002/1522-2683(200105)22:7<1305::AID-ELPS1305>3.0.CO;2-Q.

DOI:10.1002/1522-2683(200105)22:7<1305::AID-ELPS1305>3.0.CO;2-Q

PMID:11379952

Abstract

Liposomes made of mixtures of zwitterionic and anionic lipids were investigated by means of capillary electrophoresis and dynamic light scattering. The influence of the molar lipid ratio and of the buffers, used in the running electrolyte solution, on the physical characteristics of the liposomes were investigated. Data on effective electrophoretic mobilities, total charges as well as sizes of the liposomes are given. In addition, examples on the use of liposomes as carriers in electrokinetic capillary electrophoresis for the separation of benzene derivatives, steroids, and phenols are shown.

摘要

通过毛细管电泳和动态光散射研究了由两性离子脂质和阴离子脂质混合物制成的脂质体。研究了运行电解质溶液中脂质摩尔比和缓冲液对脂质体物理特性的影响。给出了脂质体有效电泳迁移率、总电荷以及尺寸的数据。此外，还展示了脂质体作为电动毛细管电泳中载体用于分离苯衍生物、类固醇和酚类的实例。