Analytical and comparative chromatographic maps of oligosaccharides produced by acetolysis and partial acid hydrolysis of glycoprotids.

The authors describe a chromatographic mapping procedure of oligosaccharides present in acetolysates and partial acid hydrolysates of glycopeptides or glycoproteins. Oligosaccharides are first fractionated on charcoal-Celite columns and then identified by paper chromatography. This procedure is sensitive and reproducible and allows to compare the structure of N-glycans and of glycoproteins from various sources.