通过对带有ATP类似物的昆虫飞行肌肉进行X射线衍射研究揭示的横桥构象。

Goody R S, Holmes K C, Mannherz H G, Leigh J B, Rosenbaum G

Biophys J. 1975 Jul;15(7):687-705. doi: 10.1016/S0006-3495(75)85848-6.

The effects of three ATP analogues, alpha,beta-methylene-ATP [ATP(alpha,beta-CH1)], adenosine 5'-0-(3-thiotrophosphate) [ATP(gamma-S)], and beta,gamma-amino-ATP [ATP(beta,gamma-NH)] at various concentrations and temperatures on the X-ray fiber diagrams of glycerinated flight muscles from a water bug (Lethocerus maximus) have been investigated. It is shown that the "relaxed" state can be obtained with all three analogues at high concentrations, the result being particularly clear with ATP(gamma-S). It is inferred that the binding of an ATP-like molecule suffices to produce the relaxed state. At low concentrations ATP(beta,gamma-NH) produces state intermediate between rigor and relaxed which is not simply a mixture of the two. The possible nature of the intermediate is discussed.

研究了三种ATP类似物，即α,β-亚甲基-ATP [ATP(α,β-CH1)]、腺苷5'-O-(3-硫代磷酸酯) [ATP(γ-S)]和β,γ-氨基-ATP [ATP(β,γ-NH)]在不同浓度和温度下对水蝽（大负子蝽）甘油化飞行肌X射线纤维图的影响。结果表明，在高浓度下，这三种类似物均可使肌肉达到“松弛”状态，其中以ATP(γ-S)的效果尤为明显。由此推断，类似ATP的分子结合足以产生松弛状态。在低浓度下，ATP(β,γ-NH)会产生介于僵直和松弛之间的中间状态，这种状态并非简单的两者混合。文中讨论了该中间状态的可能性质。