Llerena A, Berecz R, Norberto M J, de la Rubia A
Department of Pharmacology and Psychiatry, Faculty of Medicine, University of Extremadura, Badajoz, Spain.
J Chromatogr B Biomed Sci Appl. 2001 May 5;755(1-2):349-54. doi: 10.1016/s0378-4347(01)00056-1.
A rapid high-performance liquid chromatographic method has been developed for the simultaneous determination of the atypical antipsychotic drug clozapine and its principal metabolite, N-desmethyl clozapine in human plasma. After liquid-liquid extraction the compounds were separated in a reversed-phase column and measured by ultraviolet absorption at 230 nm. For both compounds inter-day variations were <3.8%, and, based on a plasma sample volume of 2 ml, the limits of quantification were 25 ng/ml. Analytical interference from coadministered psychoactive drugs and their metabolites was also studied, and no interference was found from the most commonly used antidepressants and antipsychotic drugs. The assay is sufficiently sensitive and easy to use for the analysis of plasma samples in human clinical trials and therapeutic drug monitoring.
已开发出一种快速高效液相色谱法，用于同时测定人血浆中非典型抗精神病药物氯氮平及其主要代谢物N-去甲基氯氮平。液液萃取后，化合物在反相柱中分离，并通过230nm处的紫外吸收进行测定。两种化合物的日间变化均<3.8%，基于2ml的血浆样本体积，定量限为25ng/ml。还研究了共同给药的精神活性药物及其代谢物的分析干扰，未发现最常用的抗抑郁药和抗精神病药物有干扰。该测定法灵敏度足够高且易于使用，可用于人体临床试验中的血浆样本分析和治疗药物监测。
Zotero 插件