Panda M R, Ghosh S, Varma T K
Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243 122, India.
Acta Vet Hung. 2000;48(2):173-82.
An ion-exchange chromatographic fraction of Taenia hydatigena metacestode was evaluated for use in the immunodiagnosis of ovine cysticercosis. Analysis of the fraction by sodium dodecyl sulphate--polyacrylamide gel electrophoresis revealed the presence of a 68 KDa protein. Antibodies against the isolated protein were detected in 7 out of 10 experimentally infected lambs. The diagnostic potential of the 68 KDa protein was further confirmed by testing sera from naturally infected post-mortem positive (PM+) and from apparently healthy groups of animals. Eighty % and 8% of animals were found positive by enzyme-linked immunosorbent assay (ELISA) in the groups of PM+ and apparently non-infected lambs, respectively.
对多头绦虫中绦期的离子交换色谱级分进行评估，以用于绵羊囊尾蚴病的免疫诊断。通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳对该级分进行分析，发现存在一种68 kDa的蛋白质。在10只经实验感染的羔羊中，有7只检测到针对分离出的蛋白质的抗体。通过检测自然感染的死后阳性（PM +）动物组和明显健康动物组的血清，进一步证实了68 kDa蛋白质的诊断潜力。在PM +组和明显未感染的羔羊组中，分别有80%和8%的动物通过酶联免疫吸附测定（ELISA）检测为阳性。
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