Lynn B D, Li X, Cattini P A, Nagy J I
Department of Physiology, Faculty of Medicine, University of Manitoba, 730 William Avenue, Winnipeg, R3E 3J7, Manitoba, Canada.
Neurosci Lett. 2001 Jun 22;306(1-2):49-52. doi: 10.1016/s0304-3940(01)01870-5.
Differentiation of PC12 cells by nerve growth factor (NGF) or fibroblast growth factor-2 (FGF2) is dependent on signaling mediated by extracellular regulated kinase (ERK). We investigated the involvement of receptor for hyaluronan mediated motility (RHAMM) in this signaling pathway. A single RHAMM 3.2 kb transcript was detected in PC12 RNA. Reverse transcriptase-polymerase chain reaction generated a 2141 bp cDNA that had identical sequence to rat brain RHAMM and showed no evidence of alternate splicing. Several RHAMM species were identified by Western blotting. Immunohistochemistry showed RHAMM localization to the cytoskeleton, neurites and growth cones. Following stimulation of PC12 cells with NGF or FGF2 RHAMM was co-immunoprecipitated by phosphorylation-specific anti-ERK antibodies, indicating a role for RHAMM in ERK signaling in PC12 cells.
神经生长因子（NGF）或成纤维细胞生长因子2（FGF2）诱导的PC12细胞分化依赖于细胞外调节激酶（ERK）介导的信号传导。我们研究了透明质酸介导的运动受体（RHAMM）在该信号通路中的作用。在PC12 RNA中检测到单一的3.2 kb RHAMM转录本。逆转录聚合酶链反应产生了一个2141 bp的cDNA，其序列与大鼠脑RHAMM相同，且未发现可变剪接的证据。通过蛋白质免疫印迹法鉴定出了几种RHAMM蛋白。免疫组织化学显示RHAMM定位于细胞骨架、神经突和生长锥。用NGF或FGF2刺激PC12细胞后，磷酸化特异性抗ERK抗体可共免疫沉淀RHAMM，表明RHAMM在PC12细胞的ERK信号传导中发挥作用。
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