Influence of swim-up method on the recovery of spermatozoa from different types of semen samples. - Suppr | 超能文献

Influence of swim-up method on the recovery of spermatozoa from different types of semen samples.

作者信息

Adiga S K, Kumar P

机构信息

Manipal Assisted Reproduction Centre, Kasturba Medical College, Manipal 576 119, India.

出版信息

J Assist Reprod Genet. 2001 Mar;18(3):160-4. doi: 10.1023/a:1009464121194.

DOI:10.1023/a:1009464121194

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3455597/

Abstract

PURPOSE

To compare the effectiveness of swim-up method, using different types of semen samples.

METHODS

In this retrospective study undertaken in university medical college infertility centre, subfertile couples undergoing Intra Uterine Insemination were selected. A total of 600 semen samples used for the preparation of sperm samples using swim-up method were analyzed. Relative Yield was calculated from the sperm count and motility before and after swim up from each semen sample in six different groups.

RESULT(S): Statistically significant increase in relative yield was found in oligospermic samples (20.41) followed by teratospermia (16.98). However, relative yield was low in asthenospermic (11.97) and normal (> 60 million/ml) samples (11.66).

CONCLUSION(S): Semen samples with good sperm count resulted in poor recovery after swim up. Swim-up method appears to be effective for oligospermic samples. Modifications like multiple tube swim up, using appropriate incubation time based on the initial semen parameter, will enhance the sperm yield after swim up.

摘要

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本文引用的文献

1

Evaluation of three substitutes for Percoll in sperm isolation by density gradient centrifugation.

Hum Reprod. 1998 Nov;13(11):3139-43. doi: 10.1093/humrep/13.11.3139.

2

Factors affecting sperm yield during swim-up.

J Assist Reprod Genet. 1993 Feb;10(2):145-50. doi: 10.1007/BF01207738.

3

Improved separation of motile sperm in asthenospermia and its application to artificial insemination homologous (AIH).

Fertil Steril. 1981 Aug;36(2):219-21. doi: 10.1016/s0015-0282(16)45682-7.

4

A rapid method for the fractionation of human spermatozoa according to their progressive motility.

Fertil Steril. 1981 Jun;35(6):662-5. doi: 10.1016/s0015-0282(16)45561-5.

5

Comparison of techniques for selection of motile spermatozoa.

Fertil Steril. 1985 Feb;43(2):268-73.

6

Improvement in the quality and fertilization potential of a human sperm population using the rise technique.

J Androl. 1987 Jan-Feb;8(1):25-33. doi: 10.1002/j.1939-4640.1987.tb02413.x.

7

Sperm wash in three culture media: maximization of motile sperm recovery during swim-up incubation.

Andrologia. 1987 Sep-Oct;19(5):579-84. doi: 10.1111/j.1439-0272.1987.tb01904.x.

8

Antibody binding patterns in infertile males and females as detected by immunobead test, gel-agglutination test, and sperm immobilization test.

Fertil Steril. 1988 Mar;49(3):487-92. doi: 10.1016/s0015-0282(16)59778-7.

9

Significance of reactive oxygen species and antioxidants in defining the efficacy of sperm preparation techniques.

J Androl. 1988 Nov-Dec;9(6):367-76. doi: 10.1002/j.1939-4640.1988.tb01067.x.

10

Washed intrauterine insemination--indications and success.

Int J Fertil. 1989 Nov-Dec;34(6):378-84.

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