Naylor P F, Little T W
Res Vet Sci. 1975 May;18(3):336-7.
A method for the separation of leucocytes from bovine blood and its separation into lymphocyte and granulocyte fractions is described. The method, involving flash lysis of the erythrocyte population, was found to yield large numbers of viable cells suitable for maintenance in tissue culture medium and hence of value in immunological studies.
本文描述了一种从牛血中分离白细胞并将其分为淋巴细胞和粒细胞组分的方法。该方法包括对红细胞群体进行快速裂解,结果发现可产生大量适合在组织培养基中培养的活细胞,因此在免疫学研究中有一定价值。