雅托巴尔病毒是一种含有奥罗普切病毒小RNA的重配病毒。

Jatobal virus is a reassortant containing the small RNA of Oropouche virus.

作者信息

Saeed M F, Wang H, Suderman M, Beasley D W, Travassos da Rosa A, Li L, Shope R E, Tesh R B, Barrett A D

机构信息

Department of Microbiology and Immunology, and Pathology, Center for Tropical Diseases, University of Texas Medical Branch, 77555-1019, Galveston, TX, USA.

出版信息

Virus Res. 2001 Sep;77(1):25-30. doi: 10.1016/s0168-1702(01)00262-3.

DOI:10.1016/s0168-1702(01)00262-3

PMID:11451484

Abstract

Jatobal (JAT) virus was isolated in 1985 from a carnivore (Nasua nasua) in Tucuruí, Pará state, Brazil and was classified as a distinct member of the Simbu serogroup of the Bunyavirus genus, family Bunyaviridae on the basis of neutralization tests. On the basis of nucleotide sequencing, we have found that the small (S) RNA of JAT virus is very similar (>95% identity) to that of Oropouche (ORO) virus, in particular, the Peruvian genotype of ORO virus. In comparison, limited nucleotide sequencing of the G2 protein gene, encoded by the middle (M) RNA, of JAT and ORO viruses, revealed relatively little identity (<66%) between these two viruses. Neutralization tests confirmed the lack of cross-reactivity between the viruses. These results suggest that JAT virus is a reassortant containing the S RNA of ORO virus. JAT virus was attenuated in hamsters compared to ORO virus suggesting that the S RNA of ORO virus is not directly involved in hamster virulence.

摘要

贾托巴尔（JAT）病毒于1985年从巴西帕拉州图库鲁伊的一种食肉动物（白鼻浣熊）中分离出来，基于中和试验，它被归类为布尼亚病毒科布尼亚病毒属辛布血清群的一个独特成员。基于核苷酸测序，我们发现JAT病毒的小（S）RNA与奥罗普切（ORO）病毒的小（S）RNA非常相似（同一性>95%），特别是ORO病毒的秘鲁基因型。相比之下，对JAT病毒和ORO病毒的中（M）RNA编码的G2蛋白基因进行的有限核苷酸测序显示，这两种病毒之间的同一性相对较低（<66%）。中和试验证实了这两种病毒之间缺乏交叉反应性。这些结果表明，JAT病毒是一种含有ORO病毒S RNA的重配病毒。与ORO病毒相比，JAT病毒在仓鼠中减毒，这表明ORO病毒的S RNA不直接参与仓鼠的毒力。