在长期暴露于低剂量率60Co伽马辐射的人群中，利用荧光原位杂交（FISH）检测染色体易位进行生物剂量测定。

Biodosimetry using chromosomal translocations measured by FISH in a population chronically exposed to low dose-rate 60Co gamma-irradiation.

作者信息

Hsieh W A, Lucas J N, Hwang J J, Chan C C, Chang W P

机构信息

Institute of Environmental Health Sciences, School of Medicine, National Yang Ming University, 155, Section 2, Lih-Non Street, Taipei, Taiwan 112.

出版信息

Int J Radiat Biol. 2001 Jul;77(7):797-804. doi: 10.1080/09553000110053873.

DOI:10.1080/09553000110053873

PMID:11454280

Abstract

PURPOSE

To evaluate the cumulative gamma-radiation personal exposure by analysing lymphocyte chromosome translocations using FISH painting and to compare FISH-derived biodoses with those derived from retrospective physical dose reconstruction in residents receiving chronic low dose-rate gamma-irradiation while living in radio-contaminated buildings.

MATERIALS AND METHODS

Chromosome translocation frequencies were evaluated by scoring 933 to 3077 metaphases under fluorescence microscope for each of the five male and four female exposed individuals after they had relocated from the radioactive environment for 34-82 months. FISH painting was conducted using kits of whole-chromosome probes for chromosomes 1, 2 and 4 in orange and 3, 5 and 6 in green and counter-stained with 4',6-diamidino-2-phenylindole (DAPI). The retrospective dose estimation termed Taiwan Cumulative Dose (TCD) was conducted by assessment using detailed information of historical exposure and the environmental radioactivity for each apartment during previous residency.

RESULTS

A total of 20 244 well-prepared metaphases were scored. Biodoses were calculated from the translocation frequencies and physical doses were estimated from detail questionnaires for each individual. The translocation frequencies measured ranged from 2.2x10(-3) to 26.8x10(-3) translocations per cell and the dose equivalent from 52.2 to 992.2mSv. A good correlation was observed between the physical and biodoses. A plot of TCD against FISH-derived doses produced D(fish) =0.65 D(TCD), when fitted by a linear model, and D(fish) = 0.53 D(TCD)+ 1.26x10(-4 ) D(2)(TCD), when fitted with a linear-quadratic model. Given the scatter in the data and the extremely small quadratic dose contribution, neither model could be ruled out.

CONCLUSION

Chromosome translocations provide a valid method of dose estimation in extremely protracted low dose-rate gamma-radiation exposure. Validation of the TCD method by FISH-measured translocations supports the use of TCD for epidemiological studies.

摘要

目的

通过使用荧光原位杂交（FISH）染色体涂染技术分析淋巴细胞染色体易位情况，评估累积γ射线个人暴露剂量，并比较居住在受放射性污染建筑物中接受慢性低剂量率γ射线照射居民的FISH衍生生物剂量与回顾性物理剂量重建得出的剂量。

材料与方法

在荧光显微镜下对5名男性和4名女性受照个体重新安置到非放射性环境34 - 82个月后，分别计数933至3077个中期分裂相来评估染色体易位频率。使用橙色全染色体探针试剂盒对1、2和4号染色体进行FISH涂染，绿色全染色体探针试剂盒对3、5和6号染色体进行FISH涂染，并用4',6 - 二脒基 - 2 - 苯基吲哚（DAPI）复染。回顾性剂量估计称为台湾累积剂量（TCD），通过使用历史暴露详细信息和之前居住期间每个公寓的环境放射性进行评估。

结果

共分析了20244个制备良好的中期分裂相。根据易位频率计算生物剂量，根据详细问卷为每个个体估计物理剂量。测量的易位频率范围为每细胞2.2×10⁻³至26.8×10⁻³个易位，剂量当量范围为52.2至992.2mSv。观察到物理剂量和生物剂量之间具有良好的相关性。当用线性模型拟合时，TCD与FISH衍生剂量的关系图得出D（fish）= 0.65D（TCD）；当用线性 - 二次模型拟合时，D（fish）= 0.53D（TCD）+ 1.26×10⁻⁴D²（TCD）。考虑到数据的离散性和极小的二次剂量贡献，两种模型均不能排除。