人干细胞在NOD/SCID小鼠体内的再增殖动力学

In vivo dynamics of human stem cell repopulation in NOD/SCID mice.

作者信息

Dick J E, Guenechea G, Gan O I, Dorrell C

机构信息

Programs in Cancer/Blood Research and Gene Therapy, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada, M5G 1X8.

出版信息

Ann N Y Acad Sci. 2001 Jun;938:184-90. doi: 10.1111/j.1749-6632.2001.tb03588.x.

DOI:10.1111/j.1749-6632.2001.tb03588.x

PMID:11458507

Abstract

Primitive human hematopoietic cells can be assayed on the basis of their ability to repopulate immune-deficient NOD/SCID mice and have been termed SCID repopulating cells (SRCs). The in vivo biological fate of individual SRCs can be tracked by following the unique retroviral insertion site in the progency of transduced SRCs. Distinct human SRCs were identified that differ in the proliferative and self-renewal capacity indicating that the primitive cell compartment is functionally heterogeneous.

摘要

原始人类造血细胞可根据其重新填充免疫缺陷型NOD/SCID小鼠的能力进行检测，并被称为严重联合免疫缺陷重建细胞（SRC）。通过追踪转导的SRC后代中独特的逆转录病毒插入位点，可以追踪单个SRC在体内的生物学命运。已鉴定出不同的人类SRC，它们在增殖和自我更新能力方面存在差异，这表明原始细胞区室在功能上是异质的。