枯草芽孢杆菌6-磷酸-3-己酮糖异构酶YckF的克隆、纯化及特性分析
Cloning, purification and characterization of the 6-phospho-3-hexulose isomerase YckF from Bacillus subtilis.
作者信息
Taylor E J, Charnock S J, Colby J, Davies G J, Black G W
机构信息
School of Sciences, University of Sunderland, Sunderland SR1 3SD, England.
出版信息
Acta Crystallogr D Biol Crystallogr. 2001 Aug;57(Pt 8):1138-40. doi: 10.1107/s090744490100748x. Epub 2001 Jul 23.
The enzyme 6-phospho-3-hexulose isomerase (YckF) from Bacillus subtilis has been prepared and crystallized in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop method at 291 K using polyethylene glycol 2000 monomethylether as precipitant. They diffract beyond 1.7 A using an in-house Cu Kalpha source and belong to either space group P6(5)22 or P6(1)22, with unit-cell parameters a = b = 72.4, c = 241.2 A, and have two molecules of YckF in the asymmetric unit.
已制备出枯草芽孢杆菌的6-磷酸-3-己酮糖异构酶（YckF），并将其结晶为适合X射线晶体学分析的形式。使用聚乙二醇2000单甲醚作为沉淀剂，通过悬滴法在291 K下培养晶体。使用内部铜Kα源，晶体的衍射分辨率超过1.7 Å，属于空间群P6(5)22或P6(1)22，晶胞参数a = b = 72.4，c = 241.2 Å，不对称单元中有两个YckF分子。
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