[硅胶室内面神经再生的实验研究：神经生长因子的影响]

[The experimental study of the facial nerve regeneration in silicone chamber: the influence of nerve growth factor].

作者信息

Liu Y, Gao B, Liang C

机构信息

First University Hospital, West China University of Medical Sciences, Chengdu 610041.

出版信息

Zhonghua Er Bi Yan Hou Ke Za Zhi. 1998 Feb;33(1):27-9.

PMID:11498884

Abstract

OBJECTIVE

To elucidate the role of NGF in the regeneration of facial nerve.

METHODS

The superior buccal division of facial nerve of adult New Zealand rabbit was transected and a nerve growth chamber created. The chamber of the experimental side was filled with NGF/normal saline and that of the control side with normal saline alone. Four and eight weeks after operation, the regenerated nerves in the chambers were dissected for histological studies.

RESULTS

Four weeks after operation, the average thickness of myelin sheath and the average number of myelinated axons were 0.779 +/- 0.475 micron, 2.024 +/- 1.999 (n = 11) in experimental group and 0.413 +/- 0.132 micron, 368 +/- 171 (n = 8) in control sides respectively. There was significant difference between the experimental sides and control sides (P < 0.05). Eight weeks after operation, the regenerated nerve appeared more mature. There were significant difference in the average diameters, the thickness of myelin sheath and the number of myelinated axons between the experimental and control sides (P < 0.05).

CONCLUSION

NGF within a silicone chamber enhanced facial nerve regeneration in New Zealand rabbits.

摘要

目的

阐明神经生长因子（NGF）在面神经再生中的作用。

方法

将成年新西兰兔的面神经上颊支切断，并制作神经生长室。实验组的生长室内填充NGF/生理盐水，对照组的生长室内仅填充生理盐水。术后4周和8周，取出生长室内的再生神经进行组织学研究。

结果

术后4周，实验组髓鞘平均厚度和有髓轴突平均数量分别为0.779±0.475微米、2.024±1.999（n = 11），对照组分别为0.413±0.132微米、368±171（n = 8）。实验组与对照组之间存在显著差异（P < 0.05）。术后8周，再生神经更成熟。实验组与对照组在平均直径、髓鞘厚度和有髓轴突数量上存在显著差异（P < 0.05）。