纯化绿茶表儿茶素对动脉平滑肌细胞收缩和增殖的抑制作用。
Inhibitory effects of purified green tea epicatechins on contraction and proliferation of arterial smooth muscle cells.
作者信息
Chen Z Y, Law W I, Yao X Q, Lau C W, Ho W K, Huang Y
机构信息
Departments of Biochemistry and Department of Physiology, Faculty of Medicine, Chinese University of Hong Kong, Shatin, Hong Kong, China.
出版信息
Acta Pharmacol Sin. 2000 Sep;21(9):835-40.
AIM
The present study was aimed to examine the vasorelaxant and antiproliferative responses to purified green tea epicatechin mixture, (-)epicatechin and (-)epigallocatechin gallate on rat arterial smooth muscle cells.
METHODS
Changes in isometric force were measured by Grass force transducer and effects on cell proliferation were evaluated using [3H]thymidine incorporation assay.
RESULTS
Epicatechin mixture, (-)epicatechin and (-)epigallocatechin gallate, which we isolated and purified from jasmine green tea, concentration-dependently, reduced the contractile response to phenylephrine in rat isolated aortic rings with (-)epigallocatechin gallate being more effective. These three agents also inhibited [3H]thymidine incorporation into DNA in cultured rat aortic smooth muscle cells in a concentration-dependent manner.
CONCLUSION
The purified epicatechin derivatives from jasmine green tea relaxed the isolated rat arteries preconstricted by phenylephrine and inhibited aortic smooth muscle cell proliferation.
目的
本研究旨在检测纯化的绿茶表儿茶素混合物、（-）表儿茶素和（-）表没食子儿茶素没食子酸酯对大鼠动脉平滑肌细胞的血管舒张和抗增殖反应。
方法
通过Grass力传感器测量等长力的变化，并使用[3H]胸苷掺入试验评估对细胞增殖的影响。
结果
我们从茉莉花绿茶中分离纯化得到的表儿茶素混合物、（-）表儿茶素和（-）表没食子儿茶素没食子酸酯，以浓度依赖的方式降低了大鼠离体主动脉环对去氧肾上腺素的收缩反应，其中（-）表没食子儿茶素没食子酸酯更有效。这三种药物还以浓度依赖的方式抑制[3H]胸苷掺入培养的大鼠主动脉平滑肌细胞的DNA中。
结论
从茉莉花绿茶中纯化得到的表儿茶素衍生物可舒张由去氧肾上腺素预收缩的离体大鼠动脉，并抑制主动脉平滑肌细胞增殖。
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