小鼠2型肝炎病毒感染小鼠：急性脑膜炎和肝炎的实验模型系统

Mouse hepatitis virus type-2 infection in mice: an experimental model system of acute meningitis and hepatitis.

作者信息

Das Sarma J, Fu L, Hingley S T, Lavi E

机构信息

Division of Neuropathology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.

出版信息

Exp Mol Pathol. 2001 Aug;71(1):1-12. doi: 10.1006/exmp.2001.2378.

DOI:10.1006/exmp.2001.2378

PMID:11502093

Abstract

Infection with mouse hepatitis virus (MHV) strain A59 produces acute hepatitis, encephalitis, and chronic demyelination in mice. However, little is known about a closely related strain, MHV-2, which is only weakly neurotropic. To better understand the molecular basis of neurotropism of MHVs, we compared the pathogenesis and genomic sequence of MHV-2 with that of MHV-A59. Intracerebral injection of MHV-2 into 4-week-old C57B1/6 mice produces acute meningitis and hepatitis without encephalitis or chronic inflammatory demyelination. Sequence comparison between MHV-2 and MHV-A59 reveals 94-98% sequence identity of the replicase gene, 83-95% sequence identity of genes 2a, 3, 5b, 6, and 7, and marked difference in the sequence of genes, 2b, 4, and 5a. This information provides the basis for further studies exploring the mechanism of viral neurotropism and virus-induced demyelination.

摘要

感染小鼠肝炎病毒（MHV）A59株会在小鼠中引发急性肝炎、脑炎和慢性脱髓鞘病变。然而，对于一种密切相关的毒株MHV - 2，人们了解甚少，该毒株仅具有微弱的嗜神经性。为了更好地理解MHV嗜神经性的分子基础，我们将MHV - 2的发病机制和基因组序列与MHV - A59进行了比较。将MHV - 2脑内注射到4周龄的C57B1/6小鼠中会引发急性脑膜炎和肝炎，但不会引发脑炎或慢性炎性脱髓鞘病变。MHV - 2与MHV - A59之间的序列比较显示，复制酶基因的序列同一性为94 - 98%，基因2a、3、5b、6和7的序列同一性为83 - 95%，而基因2b、4和5a的序列存在显著差异。这些信息为进一步研究病毒嗜神经性和病毒诱导的脱髓鞘机制提供了基础。