瑞香科植物欧瑞香茎中香豆素类和黄酮类化合物的抗菌活性评估

Antimicrobial evaluation of coumarins and flavonoids from the stems of Daphne gnidium L.

作者信息

Cottiglia F, Loy G, Garau D, Floris C, Casu M, Pompei R, Bonsignore L

机构信息

Dipartimento Farmaco Chimico Tecnologico, Facoltà di Farmacia, Università di Cagliari, Italy.

出版信息

Phytomedicine. 2001 Jul;8(4):302-5. doi: 10.1078/0944-7113-00036.

DOI:10.1078/0944-7113-00036

PMID:11515721

Abstract

The antimicrobial activity of stems methanol extract from Daphne gnidium L. collected from Sardinia (Italy) was evaluated against 6 strains of standard and clinical isolated gram (+/-) bacteria. The antimicrobial effect on two strains of fungi was also tested. The extract in toto exhibited antibacterial activity against Bacillus lentus and Escherichia coli, but was inactive against fungi. Four coumarins (daphnetin, daphnin, acetylumbelliferone, daphnoretin) and seven flavonoids (luteolin, orientin, isoorientin, apigenin-7-O-glucoside, genkwanin, 5-O-beta-D-primeverosyl genkwanine, 2,5,7,4'-tetrahydroxyisoflavanol) present in the plant extract were also investigated against the same strains of bacteria and fungi assayed for the crude extract. The most active compounds were daphnetin, genkwanin, and 2,5,7,4'-tetrahydroxyisoflavanol.

摘要

对从意大利撒丁岛采集的瑞香狼毒茎甲醇提取物针对6株标准菌株和临床分离的革兰氏（+/-）细菌的抗菌活性进行了评估。还测试了该提取物对两株真菌的抗菌效果。提取物整体对迟缓芽孢杆菌和大肠杆菌表现出抗菌活性，但对真菌无活性。还针对粗提取物检测的相同细菌和真菌菌株，对植物提取物中存在的四种香豆素（瑞香素、瑞香苷、乙酰伞形花内酯、瑞香脂素）和七种黄酮类化合物（木犀草素、东方蓼黄素、异东方蓼黄素、芹菜素 -7-O-葡萄糖苷、芫花素、5-O-β-D-樱草糖基芫花素、2,5,7,4'-四羟基异黄酮醇）进行了研究。活性最强的化合物是瑞香素、芫花素和2,5,7,4'-四羟基异黄酮醇。