慢性硬膜下血肿中的血管内皮生长因子
Vascular endothelial growth factor in chronic subdural haematomas.
作者信息
Shono T, Inamura T, Morioka T, Matsumoto K, Suzuki S O, Ikezaki K, Iwaki T, Fukui M
机构信息
Department of Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
出版信息
J Clin Neurosci. 2001 Sep;8(5):411-5. doi: 10.1054/jocn.2000.0951.
OBJECTIVE
To elucidate molecular aspects of the mechanisms of expansion of chronic subdural haematomas (CSH), we examined the expression of two representative angiogenic factors, vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in CSH.
METHODS
We quantified VEGF and bFGF in haematoma fluid and serum of 20 patients with CSH using an enzyme-linked immunosorbent assay. Mean concentrations of VEGF in the haematoma fluid (10277 pg/ml) and in serum, (355 pg/ml) were much greater than those of bFGF (haematoma, 3.04 pg/ml; serum, 4.74 pg/ml). Surgical specimens, including dura and the outer membrane of the CSH were analysed by in situ hybridisation to detect VEGF mRNA. Macrophages and vascular endothelial cells in the outer membrane over expressed VEGF mRNA.
CONCLUSIONS
Enhanced production of VEGF by macrophages and vascular endothelial cells in the outer membrane is thought to be pathogenetically important in CSH.
目的
为阐明慢性硬膜下血肿（CSH）扩大机制的分子层面，我们检测了两种代表性血管生成因子，即血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（bFGF）在CSH中的表达。
方法
我们采用酶联免疫吸附测定法对20例CSH患者血肿液和血清中的VEGF和bFGF进行定量。血肿液中VEGF的平均浓度（10277 pg/ml）和血清中VEGF的平均浓度（355 pg/ml）远高于bFGF（血肿液中为3.04 pg/ml；血清中为4.74 pg/ml）。通过原位杂交分析包括硬脑膜和CSH外膜在内的手术标本，以检测VEGF mRNA。外膜中的巨噬细胞和血管内皮细胞VEGF mRNA表达上调。
结论
外膜中巨噬细胞和血管内皮细胞VEGF产生增加被认为在CSH发病机制中具有重要作用。
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