食管鳞状细胞癌中14q12-q13区域扩增子内靶基因的鉴定。

Identification of target genes within an amplicon at 14q12-q13 in esophageal squamous cell carcinoma.

作者信息

Yasui K, Imoto I, Fukuda Y, Pimkhaokham A, Yang Z Q, Naruto T, Shimada Y, Nakamura Y, Inazawa J

机构信息

Department of Molecular Cytogenetics, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.

出版信息

Genes Chromosomes Cancer. 2001 Oct;32(2):112-8. doi: 10.1002/gcc.1172.

DOI:10.1002/gcc.1172

PMID:11550278

Abstract

Comparative genomic hybridization studies have revealed frequent amplification of the 14q12-q13 region in esophageal squamous cell carcinoma (ESC) cell lines. To identify genes targeted for amplification, we first defined the minimal common region of amplification using fluorescence in situ hybridization in affected ESC cell lines. The amplicon covered about 6 Mb, between markers D14S1034 and L18528. Then we screened 32 ESC cell lines to discern amplifications and expression levels of 26 expressed sequence tags (ESTs) that had been localized to the amplified region. Five known genes (BAZ1A, SRP54, NFKBIA, MBIP, and HNF3A) and two uncharacterized ESTs (GenBank Accession numbers AA991861 and AA167732) within the amplicon showed amplification and consequent overexpression. Two of these transcripts were amplified in three of the primary ESCs we examined. Our findings suggest that these seven genes are candidate targets of the amplification mechanism and therefore may be associated, together or separately, with development and progression of ESC.

摘要

比较基因组杂交研究显示，食管鳞状细胞癌（ESC）细胞系中14q12 - q13区域频繁扩增。为了鉴定扩增的靶向基因，我们首先在受影响的ESC细胞系中使用荧光原位杂交确定扩增的最小共同区域。扩增子覆盖了标记D14S1034和L18528之间约6 Mb的区域。然后我们筛选了32个ESC细胞系，以识别已定位到扩增区域的26个表达序列标签（EST）的扩增和表达水平。扩增子内的5个已知基因（BAZ1A、SRP54、NFKBIA、MBIP和HNF3A）以及2个未鉴定的EST（GenBank登录号AA991861和AA167732）显示出扩增及随后的过表达。在我们检测的三个原发性ESC中，其中两个转录本发生了扩增。我们的研究结果表明，这七个基因是扩增机制的候选靶点，因此可能共同或分别与ESC的发生和发展相关。