Maple P A, Jones C S, Andrews N J
Public Health Laboratory, Myrtle Road, Bristol BS2 8EL, UK.
J Clin Pathol. 2001 Oct;54(10):812-5. doi: 10.1136/jcp.54.10.812.
A time resolved fluorometric immunoassay (TRFIA) has been developed and compared with an in house enzyme linked immunosorbent assay (ELISA) and commercial ELISA (Bindazyme) for the detection of tetanus antitoxin in human sera. A panel of 132 sera submitted for routine testing was used. Scatterplots showed a high degree of correlation between all three assays, although some divergence of results was apparent for low titre sera when comparing in house ELISA results with Bindazyme ELISA and TRFIA results. The TRFIA appeared to be more sensitive than the in house ELISA, and the Bindazyme assay compared well with the TRFIA. The intra-assay precision of all three assays, in terms of percentage coefficient of variation (%CV), was between 2.0% and 4.0%. The interassay precision ranged from 5% to 8% for the in house ELISA, 13% to 19% for the Bindazyme assay, and 11% to 13% for TRFIA. Both Bindazyme and TRFIA assays were simple to perform, accurate, reproducible, and amenable to automation. A particular benefit of the TRFIA was its large dynamic range, enabling tetanus antitoxin values of 0.01 IU/ml to 50 IU/ml to be measured with just one dilution of serum. TRFIA appears to be a useful serological technique worthy of further development.
已开发出一种时间分辨荧光免疫分析（TRFIA）方法，并将其与内部酶联免疫吸附测定（ELISA）和商业ELISA（Bindazyme）进行比较，用于检测人血清中的破伤风抗毒素。使用了一组提交进行常规检测的132份血清。散点图显示所有三种检测方法之间具有高度相关性，尽管在将内部ELISA结果与Bindazyme ELISA和TRFIA结果进行比较时，低滴度血清的结果存在一些明显差异。TRFIA似乎比内部ELISA更敏感，Bindazyme检测与TRFIA相比表现良好。就变异系数百分比（%CV）而言，所有三种检测方法的批内精密度在2.0%至4.0%之间。内部ELISA的批间精密度为5%至8%，Bindazyme检测为13%至19%，TRFIA为11%至13%。Bindazyme和TRFIA检测方法操作简单、准确、可重复且适合自动化。TRFIA的一个特别优点是其动态范围大，只需对血清进行一次稀释即可测量0.01 IU/ml至50 IU/ml的破伤风抗毒素值。TRFIA似乎是一种值得进一步开发的有用血清学技术。
