喀麦隆的人类白细胞抗原-DR和-DQ多态性

HLA-DR and -DQ polymorphism in Cameroon.

作者信息

Pimtanothai N, Hurley C K, Leke R, Klitz W, Johnson A H

机构信息

Department of Microbiology and Immunology, Georgetown University Medical Center, 3900 Reservoir Road NW, Washington, D.C. 20007, USA.

出版信息

Tissue Antigens. 2001 Jul;58(1):1-8. doi: 10.1034/j.1399-0039.2001.580101.x.

DOI:10.1034/j.1399-0039.2001.580101.x

PMID:11580849

Abstract

HLA-DRB1, -DQA1 and -DQB1 allele frequencies were determined by high-resolution polymerase chain reaction using sequence-specific oligonucleotide probes (PCR-SSOP) and/or DNA sequencing in 126 healthy individuals in Cameroon. Eighteen DRB1, 11 DQA1, and 18 DQB1 alleles were observed. The most common alleles at each locus were DRB11503 (29%), DRB11301 (13%); DQA10102 (38%), DQA10302 (11%), DQA10401 (11%); and DQB10602 (31%), DQB10301 (13%), DQB10501 (12%). Forty-four different haplotypes were identified including 12 novel haplotypes demonstrating the HLA class II diversity resulting from allele combinations in this population. A single predominant DRB11503-DQA10102-DQB1*0602 haplotype was observed with a frequency of 27%. In summary, this study of HLA class II polymorphism in Cameroon demonstrates the extent of diversity in this population.

摘要

在喀麦隆的126名健康个体中，采用序列特异性寡核苷酸探针的高分辨率聚合酶链反应（PCR-SSOP）和/或DNA测序法确定了HLA-DRB1、-DQA1和-DQB1等位基因频率。共观察到18个DRB1等位基因、11个DQA1等位基因和18个DQB1等位基因。每个基因座最常见的等位基因分别为DRB11503（29%）、DRB11301（13%）；DQA10102（38%）、DQA10302（11%）、DQA10401（11%）；以及DQB10602（31%）、DQB10301（13%）、DQB10501（12%）。共鉴定出44种不同的单倍型，其中包括12种新单倍型，这显示了该人群中由等位基因组合导致的HLA II类分子多样性。观察到一种主要的DRB11503-DQA10102-DQB1*0602单倍型，其频率为27%。总之，这项关于喀麦隆HLA II类多态性的研究展示了该人群中的多样性程度。