重组人乙酰胆碱酯酶在转基因番茄植株中的表达。
Expression of recombinant human acetylcholinesterase in transgenic tomato plants.
作者信息
Mor T S, Sternfeld M, Soreq H, Arntzen C J, Mason H S
机构信息
Boyce Thompson Institute for Plant Research Inc., Cornell University, Tower Road, Ithaca, New York, USA.
出版信息
Biotechnol Bioeng. 2001 Nov 5;75(3):259-66. doi: 10.1002/bit.10012.
Enzyme therapy for the prevention and treatment of organophosphate poisoning depends on the availability of large amounts of cholinesterases. Transgenic plants are being evaluated for their efficiency and cost-effectiveness as a system for the bioproduction of therapeutically valuable proteins. Here we report production of a recombinant isoform of human acetylcholinesterase in transgenic tomato plants. Active and stable acetylcholinesterase, which retains the kinetic characteristics of the human enzyme, accumulated in tomato plants. High levels of specific activity were registered in leaves (up to 25 nmol min(-1) mg protein(-1)) and fruits (up to 250 nmol min(-1) mg protein(-1)).
用于预防和治疗有机磷中毒的酶疗法依赖于大量胆碱酯酶的可得性。转基因植物作为一种生产具有治疗价值蛋白质的系统，其效率和成本效益正在得到评估。在此，我们报告了在转基因番茄植株中生产人乙酰胆碱酯酶的重组同工型。具有活性且稳定的乙酰胆碱酯酶保留了人源酶的动力学特性，在番茄植株中积累。在叶片（高达25 nmol min⁻¹ mg蛋白质⁻¹）和果实（高达250 nmol min⁻¹ mg蛋白质⁻¹）中检测到高水平的比活性。
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