[西洋参中的水溶性人参皂苷]

[Water-soluble ginsenosides in American ginseng].

作者信息

Zhou Y, Song F, Liu S, Li X

机构信息

Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022.

出版信息

Zhongguo Zhong Yao Za Zhi. 1998 Sep;23(9):551-2, inside back cover.

PMID:11599389

Abstract

OBJECTIVE

To have further comprehension of American Ginseng on the basis of its water-soluble components.

METHOD

The water suspension of 80% methanol extract of American Ginseng was sequentially extracted with ethyl ether and n-butyl alcohol. The saccharides in the water-soluble portion were removed by macro-reticular resin column, and three compounds were obtained by silica-gel column chromatography. Their structures were confirmed on the basis of spectral analysis(IR, NMR, MALDI-MS) melting points and optical degrees.

RESULT

Three compounds were identified as malonyl ginsenosides Rb1, ginsenoside Rb1 and Re respectively. Among them Rb1 and Re are known, while malonyl ginsenoside Rb1 was isolated from American Ginseng for the first time. Its structure was elucidated as (3 beta, 12 beta)-20-[(-6-O-beta-D- glucopyranosyl-beta-D-glucopyranosyl)oxy]-12-hydroxylammar-24-en-3-yl-O- [6-O-(carboxyacetyl)-beta-D-glucopyranosyl)]-beta-D-glucopyranoside.

CONCLUSION

Water-soluble ginsenosides were isolated from the water-soluble extract of American Ginseng suggesting that malonyl ginsenosides exist both in American Ginseng and Ginseng.

摘要

目的

基于西洋参的水溶性成分进一步了解西洋参。

方法

西洋参80%甲醇提取物的水悬浮液依次用乙醚和正丁醇萃取。水溶性部分的糖类通过大孔树脂柱去除，经硅胶柱色谱法得到三种化合物。根据光谱分析（红外光谱、核磁共振光谱、基质辅助激光解吸电离质谱）、熔点和旋光度确定其结构。

结果

三种化合物分别鉴定为丙二酰人参皂苷Rb1、人参皂苷Rb1和人参皂苷Re。其中Rb1和Re为已知化合物，而丙二酰人参皂苷Rb1首次从西洋参中分离得到。其结构被阐明为(3β,12β)-20-[(-6-O-β-D-吡喃葡萄糖基-β-D-吡喃葡萄糖基)氧基]-12-羟基达玛-24-烯-3-基-O-[6-O-(羧基乙酰基)-β-D-吡喃葡萄糖基)]-β-D-吡喃葡萄糖苷。