多嘧啶序列结合蛋白在乙型肝炎病毒转录后调控元件功能中的作用
Role of polypyrimidine tract binding protein in the function of the hepatitis B virus posttranscriptional regulatory element.
作者信息
Zang W Q, Li B, Huang P Y, Lai M M, Yen T S
机构信息
Department of Pathology, VA Medical Center 113B, University of California School of Medicine, San Francisco, 94121, USA.
出版信息
J Virol. 2001 Nov;75(22):10779-86. doi: 10.1128/JVI.75.22.10779-10786.2001.
Abstract
The hepatitis B virus posttranscriptional regulatory element (PRE) is an RNA element that increases the expression of unspliced mRNAs, apparently by facilitating their export from the nucleus. We have identified a cellular protein that binds to the PRE as the polypyrimidine tract binding protein (PTB), which shuttles rapidly between the nucleus and the cytoplasm. Mutants of the PRE with mutations in PTB binding sites show markedly decreased activity, while cells that stably overexpress PTB show increased PRE-dependent gene expression. Export of PTB from the nucleus, like PRE function, is blocked by a mutant form of Ran binding protein 1 but not by leptomycin B. Therefore, PTB is important for PRE activity and appears to function as an export factor for PRE-containing mRNAs.
摘要
乙肝病毒转录后调控元件（PRE）是一种RNA元件，它可增加未剪接mRNA的表达，显然是通过促进其从细胞核输出实现的。我们已鉴定出一种与PRE结合的细胞蛋白，即多嘧啶序列结合蛋白（PTB），它在细胞核和细胞质之间快速穿梭。PTB结合位点发生突变的PRE突变体活性显著降低，而稳定过表达PTB的细胞中PRE依赖的基因表达增加。与PRE功能一样，PTB从细胞核的输出被Ran结合蛋白1的一种突变形式所阻断，但不被放线菌素B阻断。因此，PTB对PRE活性很重要，并且似乎作为含PRE的mRNA的输出因子发挥作用。
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