用二苦胺和苦味酸萃取分光光度法测定某些吩噻嗪类药物

Extractive-spectrophotometric determination of some phenothiazines with dipicrylamine and picric acid.

作者信息

Regulska Ewa, Tarasiewicz Mikołaj, Puzanowska-Tarasiewicz Helena

机构信息

Department of Chemistry, Technical University of Bialystok, Zamenhofa 29, 15-435, Bialystok, Poland.

出版信息

J Pharm Biomed Anal. 2002 Jan 1;27(1-2):335-40. doi: 10.1016/s0731-7085(01)00520-9.

DOI:10.1016/s0731-7085(01)00520-9

PMID:11682241

Abstract

Dipicrylamine and picric acid have been tested as reagents for the determination of promethazine and perphenazine. They react in neutral media with these drugs forming the coloured compounds. The compounds are sparingly soluble in water and quantitatively extracted into organic solvents. The extracts are intensely coloured and very stable. These properties have been exploited for the extractive spectrophotometric determination of promethazine and perphenazine in pure solutions and pharmaceuticals. Linear calibration graphs were obtained in the concentration range 4-40, 3-30 microg ml(-1) of promethazine and 4-80, 8-60 microg ml(-1) of perphenazine for picric acid and dipicrylamine, respectively. The relative standard deviation (RSD) is less than 0.8%.

摘要

已对二苦胺和苦味酸作为测定异丙嗪和奋乃静的试剂进行了测试。它们在中性介质中与这些药物反应，形成有色化合物。这些化合物微溶于水，可定量萃取到有机溶剂中。萃取液颜色很深且非常稳定。利用这些特性，采用萃取分光光度法对纯溶液和药物中的异丙嗪和奋乃静进行测定。对于苦味酸和二苦胺，分别在异丙嗪浓度范围为4 - 40、3 - 30 μg ml⁻¹以及奋乃静浓度范围为4 - 80、8 - 60 μg ml⁻¹时获得了线性校准曲线。相对标准偏差（RSD）小于0.8%。