Eap蛋白类似物在临床金黄色葡萄球菌分离株中保守且普遍存在。
Analogs of Eap protein are conserved and prevalent in clinical Staphylococcus aureus isolates.
作者信息
Hussain M, Becker K, von Eiff C, Peters G, Herrmann M
机构信息
Institute of Medical Microbiology, University of Muenster, Muenster, Germany.
出版信息
Clin Diagn Lab Immunol. 2001 Nov;8(6):1271-6. doi: 10.1128/CDLI.8.6.1271-1276.2001.
Abstract
Map and Eap are secreted Staphylococcus aureus proteins that interact with various extracellular matrix molecules. PCR analysis using map primers yielded positive reactions in 97.9% of S. aureus isolates but not in Staphylococcus epidermidis isolates. Cloning and sequencing of the conferring genes revealed a high degree of overall homology combined with size variability of the gene product due to various repeat numbers and early translation termination in a poly(A) region. Thus, Map and Eap may provide a potential novel tool for S. aureus identification and typing.
摘要
Map和Eap是金黄色葡萄球菌分泌的蛋白质，可与多种细胞外基质分子相互作用。使用map引物进行的PCR分析在97.9%的金黄色葡萄球菌分离株中产生了阳性反应，但在表皮葡萄球菌分离株中未产生阳性反应。赋予基因的克隆和测序显示，由于重复次数不同和聚腺苷酸（poly(A)）区域的早期翻译终止，基因产物在整体上具有高度同源性，同时存在大小变异。因此，Map和Eap可能为金黄色葡萄球菌的鉴定和分型提供一种潜在的新工具。
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