Croker K P, Robinson T J, Shelton J N
J Reprod Fertil. 1975 Jun;43(3):405-10. doi: 10.1530/jrf.0.0430405.
Thirty spayed ewes were used in a 2 x 3 split-plot factorial experiment (n = 5) in which oestrus was induced with 30 or 90 mug oestradiol benzoate (OB) following a period of progesterone priming. They were inseminated 36 hr after oestrogen and the numbers of spermatozoa in the caudal, mid- and cranial regions of the cervix (sub-plots) were estimated 1, 12 and 24 hr later. At each interval of time and for each region of the cervix, fewer spermatozoa were recovered from the ewes treated with 30 mug OB than from those treated with 90 mug (P less than 0-05).
30只经卵巢切除的母羊用于一项2×3裂区析因试验(n = 5),其中在经过一段孕酮预处理期后,分别用30微克或90微克苯甲酸雌二醇(OB)诱导发情。在雌激素处理后36小时进行授精,并在1小时、12小时和24小时后估计子宫颈尾段、中段和头段(裂区)的精子数量。在每个时间间隔以及子宫颈的每个区域,用30微克OB处理的母羊回收的精子比用90微克处理的母羊少(P < 0.05)。