使用两种抗体检测法和基因组分型法测定丙型肝炎病毒基因型。

Determination of HCV genotype using two antibody assays and genome typing.

作者信息

Sandres K, Dubois M, Pasquier C, Puel J, Izopet J

机构信息

Service de Virologie, Hĵpital PURPAN, Toulouse, France.

出版信息

Eur J Clin Microbiol Infect Dis. 2001 Sep;20(9):666-9. doi: 10.1007/s100960100576.

DOI:10.1007/s100960100576

PMID:11714052

Abstract

Two serotyping assays for hepatitis C virus (Serotyping 1-6 assay; Murex, UK and RIBA Serotyping SI; Chiron, USA) were compared to a standardized genotyping assay (Inno-LiPA HCV II; Innogenetics, Belgium) using serum samples collected from 126 patients chronically infected with hepatitis C virus. Serotyping was positive in 87% and 80% of the samples tested with Murex and Chiron, respectively, and concordant with genotyping in 93% and 88%, respectively. Sequence analysis of the NS5b region of 15 samples with discrepant typing results confirmed the Inno-LiPA finding in all instances. The Murex enzyme immunoassay serotyping method was less sensitive for identifying genotype 2 (P<0.05). The concordance with genotyping of the RIBA serotyping method was lower for genotype 2 than for the other genotypes (P<0.05).

摘要

将两种丙型肝炎病毒血清分型检测方法（血清分型1 - 6检测法；英国Murex公司和RIBA血清分型SI；美国Chiron公司）与一种标准化基因分型检测方法（Inno - LiPA HCV II；比利时Innogenetics公司）进行比较，使用从126例慢性丙型肝炎病毒感染患者采集的血清样本。分别用Murex和Chiron检测的样本中，血清分型阳性率分别为87%和80%，与基因分型的一致性分别为93%和88%。对15例分型结果不一致的样本的NS5b区域进行序列分析，在所有情况下均证实了Inno - LiPA的结果。Murex酶免疫分析血清分型方法在鉴定2型基因型时敏感性较低（P<0.05）。RIBA血清分型方法与基因分型的一致性在2型基因型中低于其他基因型（P<0.05）。