来自麻花头属植物麻花头（菊科）汁液中的植物蜕皮甾体。
Phytoecdysteroids from the juice of Serratula coronata L. (Asteraceae).
作者信息
Odinokov V N, Galyautdinov I V, Nedopekin D V, Khalilov L M, Shashkov A S, Kachala V V, Dinan L, Lafont R
机构信息
Institute of Petrochemistry and Catalysis, Bashkortostan Republic Academy of Sciences and the Ufa Scientific Centre of the Russian Academy of Sciences, 450075, Ufa, prosp. Oktyabrya 141, Russia.
出版信息
Insect Biochem Mol Biol. 2002 Feb;32(2):161-5. doi: 10.1016/s0965-1748(01)00106-0.
Seven phytoecdysteroids have been isolated from Serratula coronata L. One of them is a new phytoecdysteroid, 3-epi-20-hydroxyecdysone. Two further ecdysteroids, 20-hydroxyecdysone 22-acetate and taxisterone, are isolated from this species for the first time in addition to the typical S. coronata ecdysteroids, 20-hydroxyecdysone, ecdysone, ajugasterone C and polypodine B. The juice squeezed from aerial parts of fresh plants of S. coronata was extracted with ethyl acetate. The ecdysteroids were isolated by a combination of chromatographic techniques (mainly HPLC) and identified by 1D and 2D (1)H and (13)C NMR experiments and mass-spectrometry. The biological activities of 3-epi-20-hydroxyecdysone (EC(50)=1.6 x 10(-7) M), taxisterone (EC(50)=9.5 x 10(-8) M) and ajugasterone C (EC(50)=6.2 x 10(-8) M) have been determined in the Drosophila melanogaster B(II) bioassay for ecdysteroid agonist activity.
已从麻花头属植物中分离出七种植物蜕皮甾类化合物。其中一种是新的植物蜕皮甾类化合物，3-表-20-羟基蜕皮激素。除了典型的麻花头属蜕皮甾类化合物20-羟基蜕皮激素、蜕皮激素、筋骨草甾酮C和多足蕨素B之外，还首次从该物种中分离出另外两种蜕皮甾类化合物，20-羟基蜕皮激素22-乙酸酯和羟基促蜕皮甾酮。用乙酸乙酯萃取新鲜麻花头属植物地上部分挤出的汁液。通过色谱技术（主要是高效液相色谱法）的组合分离蜕皮甾类化合物，并通过一维和二维氢核磁共振实验以及质谱法进行鉴定。已在黑腹果蝇B（II）生物测定中测定了3-表-20-羟基蜕皮激素（半数有效浓度=1.6×10⁻⁷ M）、羟基促蜕皮甾酮（半数有效浓度=9.5×10⁻⁸ M）和筋骨草甾酮C（半数有效浓度=6.2×10⁻⁸ M）的蜕皮甾类激动剂活性。
Zotero 插件