上海汉族人群中α1-抗糜蛋白酶与载脂蛋白E基因多态性与帕金森病的关联研究

[Association between polymorphism of alpha 1-antichymotrypsin and apolipoprotein E gene and Parkinson's disease in Shanghai Hans].

作者信息

Hao Y, Xie H, Xu L

机构信息

Department of Neurology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2001 Oct;81(19):1172-5.

PMID:11769703

Abstract

OBJECTIVE

To explore the relationship between polymorphism of alpha 1-antichymotrypsin(AACT) and apolipoprotein E(ApoE) genes and the risk of Parkinson's disease(PD) in Shanghai Hans.

METHODS

Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were used to study the distribution of AACT and ApoE gene polymorphism in 64 PD cases and 101 healthy elderly persons as controls. Association analysis was performed using odds ratio (OR) and chi 2 test.

RESULTS

No difference in distribution of AACT A/T allele polymorphism was found between PD cases and healthy controls(chi 2 = 0.83 P = 0.363 > 0.05), but AACT-AA genotype was associated with PD(OR = 2.68, chi 2 = 4.31 P = 0.038 < 0.05). The ApoE gene polymorphism was not associated with PD(allele: chi 2 = 2.85 P = 0.241 > 0.05; genotype: chi 2 = 9.25 P = 0.099 > 0.05). The AACT-AT genotype frequency in PD patients was significantly lower than that in the controls without ApoE epsilon 4 allele(OR = 0.47, chi 2 = 4.10 P = 0.043 < 0.05). In AACT-AT genotype the ApoE epsilon 4 allele was associated with PD(OR = 3.26, chi 2 = 5.53 P = 0.019 < 0.05).

CONCLUSION

The AACT-AA genotype and AACT-AT genotype with ApoE epsilon 4 might be susceptible factors for PD in Shanghai Hans.

摘要

目的

探讨上海汉族人群中α1-抗糜蛋白酶（AACT）基因多态性和载脂蛋白E（ApoE）基因多态性与帕金森病（PD）发病风险的关系。

方法

采用聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）技术，研究64例PD患者和101例健康老年人（作为对照）中AACT和ApoE基因多态性的分布情况。采用比值比（OR）和卡方检验进行关联分析。

结果

PD患者与健康对照之间AACT A/T等位基因多态性分布无差异（卡方=0.83，P=0.363>0.05），但AACT-AA基因型与PD相关（OR=2.68，卡方=4.31，P=0.038<0.05）。ApoE基因多态性与PD无关（等位基因：卡方=2.85，P=0.241>0.05；基因型：卡方=9.25，P=0.099>0.05）。PD患者中AACT-AT基因型频率显著低于无ApoEε4等位基因的对照组（OR=0.47，卡方=4.10，P=0.043<0.05）。在AACT-AT基因型中，ApoEε4等位基因与PD相关（OR=3.26，卡方=5.53，P=0.019<0.05）。