影响新生小鼠角质形成细胞和成纤维细胞上Fas及Fas配体表达的因素

Factors influencing the expression of Fas and Fas ligand on newborn murine keratinocytes and fibroblasts.

作者信息

He W, Zhao Y, Song F, Saitoh A, Shimada S

机构信息

Department of Dermatology, The First Clinical College, China Medical University, Shenyang 110001, China.

出版信息

Chin Med J (Engl). 2000 Sep;113(9):833-5.

PMID:11776081

Abstract

OBJECTIVE

To investigate the expression of Fas, FasL mRNA and proteins on keratinocytes and fibroblasts, and the influence by retinoid acid (RA) and dexamethasone (Dex).

METHODS

RNase protection assay and flow cytometry were used.

RESULTS

Fas mRNA was expressed on neonatal murine keratinocytes and was up-regulated by all-trans retinoid acid and down-regulated by Dex. RA and Dex also increased the Fas protein expression on keratinocytes. Newborn murine keratinocytes express FasL mRNA, which was up-regulated by RA and Dex, while its protein expression was moderately induced by RA. Newborn murine fibroblasts also expressed Fas and FasL mRNA, which were up-regulated by the RA and Dex.

CONCLUSIONS

The results indicated that RA and Dex might regulate apoptosis of keratinocytes and fibroblasts via the Fas-FasL system.

摘要

目的

研究角质形成细胞和成纤维细胞上Fas、FasL mRNA及蛋白的表达情况，以及维甲酸（RA）和地塞米松（Dex）对其的影响。

方法

采用核糖核酸酶保护试验和流式细胞术。

结果

Fas mRNA在新生小鼠角质形成细胞上表达，全反式维甲酸使其上调，地塞米松使其下调。RA和Dex也增加了角质形成细胞上Fas蛋白的表达。新生小鼠角质形成细胞表达FasL mRNA，RA和Dex使其上调，而其蛋白表达受到RA适度诱导。新生小鼠成纤维细胞也表达Fas和FasL mRNA，RA和Dex使其上调。