[他莫昔芬对乳腺癌细胞体外凋亡及耐药性的影响]
[Effect of tamoxifen on apoptosis and drug resistance of breast cancer cells in vitro].
作者信息
Zheng J, Yao Z
机构信息
Department of General Surgery, Clinical College, Chongqing University of Medical Sciences, Chongqing 400016, China.
出版信息
Zhonghua Zhong Liu Za Zhi. 2000 Jan;22(1):55-7.
OBJECTIVE
To study the effect of estradiol (E2) and tamoxifen (TAM) on the apoptosis regulatory genes (bax, bcl-2) and mdr-1 in MCF-7 cells.
METHODS
Immunohistochemistry, in situ hybridization were used to observe the changes in bax, bcl-2 and mdr-1 genes after treatment with E2 and TAM.
RESULTS
E2 had ability to increase the expression of bcl-2 and mdr-1 but TAM decrease the expression of both. Synthetic bax oligodeoxynucleotide (ODN) was used as probe to perform in situ hybridization. It was shown that bax mRNA in MCF-7 cells was down-regulated by E2 and up-regulated by TAM. Significant correlation between bcl-2 and mdr-1 was observed after TAM treatment of MCF-7 cells (P < 0.05).
CONCLUSION
TAM regulates cell apoptosis and inhibits development of drug-resistance of MCF-7 breast cancer.
目的
研究雌二醇（E2）和他莫昔芬（TAM）对MCF-7细胞凋亡调控基因（bax、bcl-2）及多药耐药基因1（mdr-1）的影响。
方法
采用免疫组织化学、原位杂交技术观察E2和TAM处理后bax、bcl-2和mdr-1基因的变化。
结果
E2能增加bcl-2和mdr-1的表达，而TAM则降低二者的表达。以合成的bax寡脱氧核苷酸（ODN）为探针进行原位杂交。结果显示，E2可下调MCF-7细胞中bax mRNA的表达，而TAM则上调其表达。TAM处理MCF-7细胞后，bcl-2与mdr-1之间存在显著相关性（P < 0.05）。
结论
TAM可调节细胞凋亡并抑制MCF-7乳腺癌耐药性的发展。
Zotero 插件