糖修饰的嵌段共聚物胶束：其制备及与凝集素分子的特异性相互作用。

Sugar-installed block copolymer micelles: their preparation and specific interaction with lectin molecules.

作者信息

Nagasaki Y, Yasugi K, Yamamoto Y, Harada A, Kataoka K

机构信息

Department of Materials Science, Science University of Tokyo, Noda 278-8510, Japan.

出版信息

Biomacromolecules. 2001 Winter;2(4):1067-70. doi: 10.1021/bm015574q.

DOI:10.1021/bm015574q

PMID:11777374

Abstract

Several types of sugar-installed poly(ethylene glycol)/poly(DL-lactide) (sugar-PEG/PLA) block copolymers were synthesized. The synthesized block copolymer forms a core-shell type polymeric micelle in aqueous media possessing sugar molecules on its surface. Specific recognition of lectin proteins with the sugar molecules on the micelle surface was observed. Both the galactose- and lactose-installed micelles specifically interacted with RCA-1; on the other hand the mannose-installed micelle interacted specifically with Con A. With a lectin-immobilized affinity column, the cluster effect of the sugar molecule on the micelle surface was clearly observed.

摘要

合成了几种类型的含糖聚（乙二醇）/聚（DL-丙交酯）（糖-PEG/PLA）嵌段共聚物。所合成的嵌段共聚物在水性介质中形成核壳型聚合物胶束，其表面带有糖分子。观察到凝集素蛋白与胶束表面的糖分子之间存在特异性识别。半乳糖和乳糖修饰的胶束均与RCA-1发生特异性相互作用；另一方面，甘露糖修饰的胶束与伴刀豆球蛋白A发生特异性相互作用。使用固定有凝集素的亲和柱，清晰地观察到了胶束表面糖分子的簇集效应。