基于16S-23S核糖体DNA内部转录间隔区序列鉴别鼻疽分枝杆菌和塞内加尔分枝杆菌菌株
Differentiation between Mycobacterium farcinogenes and Mycobacterium senegalense strains based on 16S-23S ribosomal DNA internal transcribed spacer sequences.
作者信息
Hamid Mohamed E, Roth Andreas, Landt Olfert, Kroppenstedt Reiner M, Goodfellow Michael, Mauch Harald
机构信息
Department of Preventive Medicine and Public Health, Faculty of Veterinary Science, University of Khartoum, Khartoum North, Sudan.
出版信息
J Clin Microbiol. 2002 Feb;40(2):707-11. doi: 10.1128/JCM.40.2.707-711.2002.
Abstract
16S ribosomal DNA (rDNA) and 16S-23S internal transcribed spacer rDNA sequence analyses were performed on Mycobacterium farcinogenes and M. senegalense strains and 26 strains of other rapidly growing mycobacteria to investigate the phylogenetic structure of bovine farcy mycobacteria within the M. fortuitum complex. M. farcinogenes and M. senegalense were indistinguishable in their 5"-end 16S rDNA but showed both considerable interspecies spacer sequence divergence and a high level of intraspecies sequence stability. A rapid detection assay using PCR and hybridization with species-specific probes was developed. The assay was specific among 46 species other than M. farcinogenes and M. senegalense and correctly identified all M. farcinogenes and M. senegalense strains. PCR- and 16S-23S rDNA sequence-based detection will be a valuable approach for diagnosis of the causal agents of African bovine farcy in cattle.
摘要
对鼻疽分枝杆菌和塞内加尔分枝杆菌菌株以及26株其他快速生长分枝杆菌进行了16S核糖体DNA（rDNA）和16S-23S内转录间隔区rDNA序列分析，以研究偶然分枝杆菌复合体内牛鼻疽分枝杆菌的系统发育结构。鼻疽分枝杆菌和塞内加尔分枝杆菌在其5'端16S rDNA中无法区分，但显示出种间间隔序列有相当大的差异以及种内序列具有高度稳定性。开发了一种使用PCR和与种特异性探针杂交的快速检测方法。该方法在除鼻疽分枝杆菌和塞内加尔分枝杆菌之外的46个物种中具有特异性，并能正确鉴定所有鼻疽分枝杆菌和塞内加尔分枝杆菌菌株。基于PCR和16S-23S rDNA序列的检测将是诊断牛非洲鼻疽病原体的一种有价值的方法。
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