Zhong M, Wang P, Wang S, Tang W, Wu Z
Department of General Surgery, Renji Hospital, Shanghai Second Medical University, Shanghai 200001.
Zhonghua Wai Ke Za Zhi. 1999 Dec;37(12):715-7.
To observe gamma delta T cells and their receptors Vdelta gene rearrangement expression in the transitional mucosa of rectal carcinoma, and to investigate their relations with local tumor recurrence.
In 13 patients with rectal carcinoma, gamma delta T cells in transitional mucosa were measured with two-color immunofluorescent flow cytometric analysis and polymerase chain reaction technique.
The volume of gamma delta T cells in transitional mucosal lymphocytes (TML) were significantly higher than that in tumor infiltrating lymphocytes (TIL), and significantly lower than that in normal intestinal epithelial lymphocytes (IEL) (P < 0.05). The gamma delta T cell receptors delta chain V region (gamma delta-TCR Vdelta1-Vdelta6) gene rearrangement was all expressed in TIL, TML and IEL; but the Vdelta 6 gene expression in both TML and TIL were higher than that in IEL (P < 0.05).
The decreased volume of gamma delta T cells in TML resulted in decreased local immune function and may be responsible for the local tumor recurrence after resection. The high expression of Vdelta 6 gene rearrangement in both TIL and TML suggests the skewing of gamma delta-TCR receptors and the abtitumor specificity of TIL and TML in local epithelium.
观察直肠癌移行黏膜中γδT细胞及其受体Vδ基因重排表达情况，探讨其与局部肿瘤复发的关系。
采用双色免疫荧光流式细胞术及聚合酶链反应技术检测13例直肠癌患者移行黏膜中的γδT细胞。
移行黏膜淋巴细胞（TML）中γδT细胞数量显著高于肿瘤浸润淋巴细胞（TIL），但显著低于正常肠上皮淋巴细胞（IEL）（P<0.05）。γδT细胞受体δ链V区（γδ-TCR Vδ1-Vδ6）基因重排在TIL、TML及IEL中均有表达；但TML及TIL中Vδ6基因表达均高于IEL（P<0.05）。
TML中γδT细胞数量减少导致局部免疫功能下降，可能是直肠癌切除术后局部肿瘤复发的原因之一。TIL及TML中Vδ6基因重排高表达提示γδ-TCR受体偏移及TIL和TML在局部上皮中的抗肿瘤特异性。
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