来自红酵母（红法夫酵母）的线性DNA质粒的克隆及核苷酸序列

Cloning and nucleotide sequence of a linear DNA plasmid from Xanthophyllomyces dendrorhous (Phaffia rhodozyma).

作者信息

Santopietro L M, Kula M R

机构信息

Institut für Enzymtechnologie, Heinrich-Heine-Universität Düsseldorf, D-52426 Jülich, Germany.

出版信息

Folia Microbiol (Praha). 2001;46(4):277-88. doi: 10.1007/BF02815614.

DOI:10.1007/BF02815614

PMID:11830937

Abstract

Extrachromosomal elements were found in a strain of X. dendrorhous, and were characterized as linear DNA forming two well defined groups, pPh1 with 3 high-copy-number molecules, pPh11 (6.9 kb), pPh12 (5.7), pPh13 (4.7), and pPh2 with 2 low-copy-number molecules, pPh21 (3.6 kb), pPh22 (3.0). A 4077 bp fragment from pPh13 was cloned in pUC18 (pDK1) and sequenced (accession no. AJ 278,424). Seven putative ORF and some possible regulator sequences were defined.

摘要

在一株红法夫酵母中发现了染色体外元件，其特征为线性DNA，形成两个明确的组，pPh1有3个高拷贝数分子，即pPh11（6.9 kb）、pPh12（5.7 kb）、pPh13（4.7 kb），pPh2有2个低拷贝数分子，即pPh21（3.6 kb）、pPh22（3.0 kb）。来自pPh13的一个4077 bp片段克隆于pUC18（pDK1）并测序（登录号AJ 278424）。确定了7个推定的开放阅读框和一些可能的调控序列。