动物克隆的最新进展与问题

Recent progress and problems in animal cloning.

作者信息

Tsunoda Y, Kato Y

机构信息

Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara, 631-8505, Japan.

出版信息

Differentiation. 2002 Jan;69(4-5):158-61. doi: 10.1046/j.1432-0436.2002.690405.x.

DOI:10.1046/j.1432-0436.2002.690405.x

PMID:11841470

Abstract

It is remarkable that mammalian somatic cell nuclei can form whole individuals if they are transferred to enucleated oocytes. Advancements in nuclear transfer technology can now be applied for genetic improvement and increase of farm animals, rescue of endangered species, and assisted reproduction and tissue engineering in humans. Since July 1998, more than 200 calves have been produced by nuclear transfer of somatic cell nuclei in Japan, but half of them were stillborn or died within several months of parturition. Morphologic abnormalities have also been observed in cloned calves and embryonic stem cell-derived mice. In this review, we discuss the present situation and problems with animal cloning and the possibility for its application to human medicine.

摘要

值得注意的是，如果将哺乳动物的体细胞核转移到去核卵母细胞中，它们能够发育成完整的个体。核移植技术的进步如今可应用于家畜的遗传改良和数量增加、濒危物种的拯救以及人类的辅助生殖和组织工程。自1998年7月以来，日本通过体细胞核移植已培育出200多头小牛，但其中一半是死产或在分娩后的几个月内死亡。在克隆小牛和胚胎干细胞衍生的小鼠中也观察到了形态学异常。在这篇综述中，我们讨论了动物克隆的现状和问题以及其应用于人类医学的可能性。