电压门控钾通道Kv2.1的新型调节亚基Kv6.3的分子克隆与特性分析

Molecular cloning and characterization of Kv6.3, a novel modulatory subunit for voltage-gated K(+) channel Kv2.1.

作者信息

Sano Yorikata, Mochizuki Shinobu, Miyake Akira, Kitada Chika, Inamura Kohei, Yokoi Hiromichi, Nozawa Katsura, Matsushime Hitoshi, Furuichi Kiyoshi

机构信息

Molecular Medicine Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co., Ltd., 21 Miyukigaoka, Ibaraki 305-8585, Tsukuba, Japan.

出版信息

FEBS Lett. 2002 Feb 13;512(1-3):230-4. doi: 10.1016/s0014-5793(02)02267-6.

DOI:10.1016/s0014-5793(02)02267-6

PMID:11852086

Abstract

We report identification and characterization of Kv6.3, a novel member of the voltage-gated K(+) channel. Reverse transcriptase-polymerase chain reaction analysis indicated that Kv6.3 was highly expressed in the brain. Electrophysiological studies indicated that homomultimeric Kv6.3 did not yield a functional voltage-gated ion channel. When Kv6.3 and Kv2.1 were co-expressed, the heteromultimeric channels displayed the decreased rate of deactivation compared to the homomultimeric Kv2.1 channels. Immunoprecipitation studies indicated that Kv6.3 bound with Kv2.1 in co-transfected cells. These results indicate that Kv6.3 is a novel member of the voltage-gated K(+) channel which functions as a modulatory subunit.

摘要

我们报告了电压门控钾离子通道的一个新成员Kv6.3的鉴定与特性分析。逆转录聚合酶链反应分析表明，Kv6.3在大脑中高度表达。电生理研究表明，同源多聚体Kv6.3不能产生功能性电压门控离子通道。当Kv6.3和Kv2.1共表达时，与同源多聚体Kv2.1通道相比，异源多聚体通道的失活速率降低。免疫沉淀研究表明，在共转染细胞中Kv6.3与Kv2.1结合。这些结果表明，Kv6.3是电压门控钾离子通道的一个新成员，作为一个调节亚基发挥作用。