台湾人、泰国人和菲律宾人基德血型多态性的分子遗传学分析。

A molecular genetic analysis of the Kidd blood group polymorphism in Taiwanese, Thais, and Filipinos.

作者信息

Liu T C, Lin S F, Chang C S, Chen T P, Chang J G

机构信息

Division of Haematology-Oncology, Department of Internal Medicine, Kaohsing Medical University Hospital, Kaohsiung, Taiwan.

出版信息

Kaohsiung J Med Sci. 2001 Nov;17(11):552-5.

PMID:11852461

Abstract

In this study, we molecularly detect the Jka/Jkb polymorphism in Taiwanese, Thais, and Filipinos with PCR-restriction enzyme digestion. We designed the primers with mutagenic bases to create two restriction enzyme cutting sites (EcoN I and Xba I) simultaneously to confirm the genetic polymorphism of Jka/Jkb. One hundred and seven unrelated Taiwanese, 107 Thais foreign laborers, and 102 Filipino foreign laborers in Taiwan were analyzed. The frequency of Jka and Jkb is 0.51 and 0.49 in Taiwanese, 0.44 and 0.56 in Thais, and 0.45 and 0.55 in Filipinos, respectively. The molecular analysis of Jka/Jkb polymorphism is simple, accurate and could confirm the uncertain results obtained by serological typing. The method could be used for predicting fetal Kidd genotype from amniocyte DNA, and to avoid the hemolytic disease of newborns caused by Jk antibody.

摘要

在本研究中，我们采用聚合酶链反应-限制性内切酶消化法对台湾人、泰国人和菲律宾人的Jka/Jkb多态性进行分子检测。我们设计了带有诱变碱基的引物，以同时创建两个限制性内切酶切割位点（EcoN I和Xba I），从而确认Jka/Jkb的基因多态性。对107名无亲缘关系的台湾人、107名泰国外籍劳工以及102名在台湾的菲律宾外籍劳工进行了分析。Jka和Jkb在台湾人中的频率分别为0.51和0.49，在泰国人中为0.44和0.56，在菲律宾人中为0.45和0.55。Jka/Jkb多态性的分子分析简单、准确，能够确认血清学分型获得的不确定结果。该方法可用于从羊水细胞DNA预测胎儿基德基因型，并避免由Jk抗体引起的新生儿溶血病。