Costanzo F, Castagnoli L, Dente L, Arcari P, Smith M, Costanzo P, Raugei G, Izzo P, Pietropaolo T C, Bougueleret L, Cimino F, Salvatore F, Cortese R
European Molecular Biology Laboratory, Heidelberg, FRG.
EMBO J. 1983;2(1):57-61. doi: 10.1002/j.1460-2075.1983.tb01380.x.
A human cDNA library was constructed using M13 derivative vectors. The simple and rapid procedures for sequencing single-stranded DNA by the dideoxy chain termination method allowed a screening of individual clones directly by DNA sequence analysis. Some of these clones were identified as coding for: serum albumin, alpha1-antitrypsin, retinol-binding protein, prothrombin, haptoglobin, and metallothionein. Furthermore, a clone coding for aldolase B was tentatively identified on the basis of high sequence homology with rabbit muscle aldolase.
使用M13衍生载体构建了人cDNA文库。通过双脱氧链终止法对单链DNA进行测序的简单快速程序，使得能够直接通过DNA序列分析筛选单个克隆。其中一些克隆被鉴定为编码以下蛋白：血清白蛋白、α1-抗胰蛋白酶、视黄醇结合蛋白、凝血酶原、触珠蛋白和金属硫蛋白。此外，基于与兔肌肉醛缩酶的高度序列同源性，初步鉴定出一个编码醛缩酶B的克隆。
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